Also as it is thin, it is hard to measure with the human eye exactly the temperature desired, which can cause inaccuracies. To make sure the water is of the correct temperature. 1 0 obj It was observed that as the temperature increases, the rate of photosynthesis increases as well. The proteins are folded into a very particular shape, and this allows them to bind efficiently to the molecules of interest. However in some water baths, even though the temperature was set, when we checked with the thermometer, it wasn’t that temperature exactly. This cycle is the Calvin cycle. Some of these triose phosphates condense to form hexose phosphates, sucrose, starch and cellulose or are converted to acetylcoenzyme A to make amino acids and lipids. This will be done by measuring the distance of the lamp from the elodea on each experiment to keep the distance equal. Depending on the particular plant in question, set the greenhouse thermostat to a temperature within this range for best results. Standard deviation of the volume of oxygen released at 35oC squared, divided by number of pieces of data = (11.36500358)2 = 9.935638952, 4. <>>> Work out the standard deviation of one set of data and square it. After 2. To investigate the effect of temperature on the rate of photosynthesis of  a Mauna Loa Supreme leaf. The water bath needs to be plugged in to electronically keep the temperature accurate. Use the hole puncher to cut out 10 or more disks from the leaves. This is not a very accurate way to keep the temperature level. Place the tube in a beaker of fresh water. The water in our experiment will be contained in the test tube the elodea is placed in, therefore it will be taken from here to be used in photosynthesis. A bicarbonate ion is one of the carbon sources for photosynthesis. Investigating Factors Affecting the Rate of Photosynthesis, University of California, Berkeley: Photosynthetic Adaptation to Temperature, Thompson Morgan: Top Ten Tips for Heating a Greenhouse in Winter. This means Maximum photosynthetic rates vary between plant species, but crops such as maize can achieve carbon dioxide assimilation rates as high as 0.075 ounce per cubic foot per hour, or 100 milligrams per decimeter per hour. At high light intensity, photosynthetic rate levels out, due to other limiting factors, including competition between oxygen and carbon dioxide for the active site on RUBP carboxylase. This means there will be different numbers of chlorophyll on each, therefore light absorption will vary between elodea, meaning different rates of photosynthesis and different amount of oxygen released as a result of this. During the experiment, the whole of the elodea was not emerged in water. Therefore in our actual experiment we will do at least ten different temperatures, in order to make the results more reliable. One reason might be that some of the enzymes of photosynthesis are working at their maximum rate. Standard deviation of the volume of oxygen released at 65oC squared, divided by number of pieces of date = (27.83292436 )2 = 2.120994181, 5. However in our preliminary we did this by counting bubbles. Be careful not to slip, therefore walk and not run in the class. In a solution that contains only extracted pigment, the absorbed energy cannot usefully be passed on to do work and electrons return to their unexcited state and the absorbed energy is transferred to the surroundings as thermal energy and as light at a longer and less energetic wavelength than that which was absorbed, this is the red fluorescence. To fit the trend line exactly, the average rate of oxygen released should be around 37mm3/min. Simple experiments carried out by scientists shows that the rate of photosynthesis is critically dependent upon variables such as temperature, pH and intensity of light. Therefore I predict the elodea will produce more oxygen bubbles at 15oC. During my experiment, we did 2 repeats for each temperature. experiment is carried in the same environment. The time taken for the correct temperature to be reached could affect the results and cause inaccuracies, which could make the volume of oxygen released more or less than it otherwise would be. Photosystem II includes a water-splitting enzyme which catalyses the breakdown of water (photolysis): Oxygen is a waste product of this process. This is due to human error, as the clock was not stopped at exactly 5 minutes. amount of water, heat plenty of water and after heating measure its mentioned in Equation 1, 2 0 obj 3. Create your own unique website with customizable templates. The hydrogen ions combine with a carrier molecule NADP to make reduced NADP. This could affect the results in that there was less photosynthesis taking place than our results show. photosynthesis requires CO2 so the more CO2  in the environment, the greater rate It takes long for the ice to melt and for it to affect the temperature. ���D��&��Y� *�-Cb_�����RX�%{��0�Y�l�bb�`K��I��m�n�$tffc��P��cw�6|iɿ;�9��#���Oݓ�����������vxk���o�f����?�럵-���R�0���N��X'\/�V�x2��'�����a%?�k���m�C͆\�M��Žry���5�֭�V][�V�c-����P5i�'6�\��l����ː��W$�\���;J{߿�D�Oy�Y=9=�"��Ý����]� u7�x��l��� Aim: The aim of this experiment is to evaluate the effect of varying the temperature on the rate of photosynthesis. * The amount of sodium bicarbonate added to the experiment is different in each experiment, as the amounts were not measured. Retrieved from, Type: After all, a conclusion ought to be come and some The sum of the figures calculated in steps 3 and 4 =, 6. Place excess sodium bicarbonate (NaHCO3) in the water to give a constant saturated solution of CO2. This is where the enzymes have the maximum amount of kinetic energy, therefore the active site and substrate will collide more, with more energy forming the product GP at the fastest possible rate. This is because the enzyme RUBISCO works best at higher temperatures as they gain more kinetic energy, therefore binding with active sites at a faster rate. The ATP then passes to the light-independent reactions. This is also slightly inaccurate as there is a significant difference. result of all these, some plants can carry on photosynthesis with a greater Photosynthesis defines the process by which plants and some bacte… As this is a factor that affects the rate of photosynthesis, this could cause inaccuracies in my results if the light intensity is varied through my experiment. this experiment, the amount of water is 100 ml (±0,5 ml) This leads to a decrease in glucose production and will result in stunted growth. 8. As The line of best-fit increases steadily from 22oC and 35oC on graph 1, where the average rate of oxygen released went from 12mm3/min to 30.8mm3/min. Cover the windows and doors in black paper. 8. This was very inaccurate as even small amounts of warm water affect the temperature by a large amount. Optimum photosynthetic rates lead to the removal of greater amounts of carbon dioxide from the local atmosphere, producing greater amounts of glucose. Start with tap water, as the temperature of this would be nearest to 15oC. <> (2020, Jun 02). This is a small difference, therefore the set of results for 0 degrees is very accurate. plant has different properties for carriyng on photosynthesis like the number So it is 6 0 obj photosynthesis reaction is taken and which is. Human error could have affected them, this is because the elodea was changed in between the days. The temperature of the water will be changed for all experiments, this will be what we will measure. Here light energy is converted to chemical energy. The difference between these results with a range of 15 degrees is 29.4mm3/min. After calculating, percentage N�� ����kؤ. %���� At low temperatures, between 32 and 50 degrees Fahrenheit – 0 and 10 degrees Celsius – the enzymes that carry out photosynthesis do not work efficiently, and this decreases the photosynthetic rate. This passes to the light-independent reactions and is used in the synthesis of carbohydrate. endobj At temperatures above 40°C the rate slows down. Respiration releases energy, of which is needed in photosynthesis. I predict that when the elodea is placed in water with a temperature of 0oC, there rate of photosynthesis will be at a minimum. The temperature needs to be monitored as it can change during the course of the experiment as it can vary slightly, causing inaccuracies in the results. The experiments took place at different times of the day, therefore there would have been different light intensities for different experiments, which would cause inaccuracies in our results. This makes the rate of the disk rise an indirect measurement of the net rate of photosynthesis. The meter was constantly fluctuating therefore it is not completely certain how constant light intensity was in each experiment. can have different number of stomas, which directly affects the rate of I correctly predicted that at 35oC the temperature would increase further as the kinetic energy rubisco has increases. ( When the leaves were at a temperature of 23o C, the carbon dioxide diminished at a rate of -0.079724 ppm/s. When carbon dioxide concentration is low less GP can be produced. For our actual experiment the room will be blacked out, therefore the light from outside will not affect the experiment. This will mean that the temperature in all experiments will stay constant, as adding ice and warm water is not accurate. On the class average graph (graph 2), there is also one anomalous result. <> 4. In Therefore we will need to be careful not to get water near the plug points, as their could be a risk of an electric shock. =8�Q�EC�RpEG�"T� x7��T7� �Ju����QP�n��=)� �w��R]��ȉO���]��GIE������"x1����EN}^E!����u����O�. The optimum temperature for enzymes in plants is around 40oC. What the results mean. This essay will explain the role of carbon dioxide in various parts of the carbon cycle.

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